all of the substrate is a "dead-spot". As Mudskippers are native to mangrove/mudflat environments, the sediments are annoxic within 2cm of the surface. In order to get these fish to breed, I had to replicate this. The substrate that I use is a very glutinous mix of fine sand, silt and mud. The sediments are not disturbed (except by the fish), and eventually and algae layer has formed which has consolidated the surface of the mud/sand mix. To maintain the slope, I have a slatted plastic barrier sitting just under the surface. This prevents movement or collapse of the mud slope. The tank has been drilled in 2 spots on the base, which has allowed me to hide the inflow and outflow tudes, and has also aided in draining the tank to maintain the high/low tide levels (ie the water does not have to siphon-out, it mearly falls with gravity.